Department of Biology

Event Information

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Title: GCDB Seminar Series: "Enzymatic modules of the SAGA chromatin-modifying complex play distinct roles in Drosophila gene expression and development." Jerry Workman.
Start Time: Thursday November 09, 2017 04:00 PM
End Time: Thursday November 09, 2017 05:00 PM
Location: Myers Hall 130  
Contact: Biology Seminar Coordinator
Description:

Abstract

The Spt-Ada-Gcn5-acetyltransferase (SAGA) chromatin-modifying complex is a transcriptional coactivator that contains four different modules of subunits. The intact SAGA complex has been well characterized for its function in transcription regulation and development. However, little is known about the roles of individual modules within SAGA and if they have any SAGA independent functions. Here we demonstrate that the two enzymatic modules of Drosophila SAGA are differently required in oogenesis. Loss of the histone acetyltransferase (HAT) activity blocks oogenesis, while loss of the H2B deubiquitinase (DUB) activity does not. However, the DUB module regulates a subset of genes in early embryogenesis and loss of the DUB subunits causes defects in embryogenesis. ChIP-seq analysis revealed that both the DUB and HAT modules bind most SAGA target genes even though many of these targets do not require the DUB module for expression. Furthermore, we found that the DUB module can bind to chromatin and regulate transcription independently of the HAT module. Our results suggest that the DUB module has functions within SAGA and independent functions.

Contact Email: biotalk@indiana.edu
More Contact Info: Host: Gabe Zentner
Other Info: Refreshments served prior to seminar.
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